Single B Cell Screening is a cutting-edge platform for discovering antigen-specific antibodies, enabling isolation, analysis, and retrieval of individual B cells with their naturally paired VH/VL genes. Fluorescence-activated cell sorting (FACS) allows precise, high-throughput selection by simultaneously labeling antigens and B cell surface markers with multiple fluorescent tags. Compared to traditional hybridoma methods, this approach delivers high-affinity, antigen-specific antibodies in as little as 8 weeks, significantly accelerating discovery timelines. Widely used in therapeutic antibody development, diagnostic screening, and research, it supports applications ranging from cancer immunotherapy and infectious disease studies to signal pathway studies and protein function validation.
Service Name | Service ID | Turnaround | Deliverables |
Single B Cell Screening Service – Mouse mAb | Tsingke-B01 | 8~10 Weeks | Phase I Collect 5 μL serum per animal for titer testing; select the best individual with titer ≥ 1:100 K to advance Phase II (1) 10 positive culture supernatants (2) 1 antibody gene sequence (3) 1–3 mg of purified mAb with corresponding expression plasmids (ELISA titer ≥ 1:100 K) |
Single B Cell Screening Service – Rabbit mAb | Tsingke-B02 |
*Turnaround time in the table applies only when the client provides antigens.
A total of 50 mouse mAbs were obtained from FACS-sorted single B cells. All bound the target antigen (100% ELISA positive) with 45 showing strong binding.
A total of 29 rabbit mAbs were obtained from FACS-sorted single B cells. All bound the target antigen (100% ELISA positive) with 18 showing strong binding.
Yes, memory B cells need to differentiate into terminal plasma cells in order to secrete antibodies.
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