Site-directed Mutagenesis enables precise introduction of specific DNA mutations, including point mutations, insertions, or deletions. It is widely applied in protein functional site studies, enzyme activity optimization, DNA element functional analysis, and gene therapy research.
Tsingke provide professional and cost-effective site-directed mutagenesis services, accurately introducing desired mutations into any gene template at any number of sites, significantly enhancing experimental efficiency and reducing research costs.
| Length | Turnaround time (Calendar Day) | Vector | Deliverables |
| < 1.5 kb | 7-10 | Customer-specific | (1) 1 tube of lyophilized plasmid DNA (about 1~4 μg/tube); (2) QC files: Sequencing map (.abl file); Target sequence (.seq file); COA Report (elec tronic); (3) Any mutations identified within a 30-base region will be considered as a single com prehensive mutation. |
| 1.5kb~3kb | 7-11 | ||
| 3kb~6kb | 10-15 | ||
| 6kb~8kb | 13-18 | ||
| ＞8kb | Evaluation |
Download the order form right and email it to info@tsingke.com.cn, or "Send Your Request" to submit your inquiry online.
a. DNA template: Please send us your template
b. Information: The sequence of the template including the target gene and vector information, mutation specifications.c.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fillin the relevant fields according to your needsand send it via email to gene@tsingke.com.cn to place your order.
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