What information do I need to provide to assess sequence pricing and turnaround time?

We require the DNA or amino acid sequence you need synthesized.If codon optimization is required, specify the host species. Additionally, indicate any required restriction sites at the 5' and/or 3' ends, any internal modifications to avoid, and if you need additional sequences added (e.g., Kozak sequence, stop codons, etc.).Or you can download the "Tsingke Gene Synthesis Order Form" from our website and email it to info@tsingke.com.cn.Our technical support team will promotly estimate the pricing and turnaround time for you and send you a quotation shortly.

What information do I need to provide for custom shRNA design?

To help us design the most effective shRNA, please provide:Gene information: Official gene name (Gene Symbol), species or Gene ID, and the target sequence.Vector preference: The vector you would like the shRNA sequence to be cloned into. You may select from our available vectors (based on selectable markers, etc.) or provide your own vector.Or you can download the "Tsingke shRNA vector construction Order Form" from our website and email it to info@tsingke.com.cn. Our technical support team will promotly estimate the pricing and turnaround time for you and send you a quotation shortly.

How to order Gene to Plasmid?

Download the "Tsingke Gene Synthesis Order Form" from our website, complete the “Gene info”, “Cloning vector”and“Plasmid preparation”section, and send it via email to info@tsingke.com.cn to place your order.

Can you keep my sequence information confidential?

Yes, all customers' information will be kept confidential.

Does Tsingke provide protein expression services after completing gene synthesis?

Yes, Tsingke also provides protein expression and purification services for synthesized genes, offering six major protein expression systems: E. coli protein expression system, Bacillus subtilis protein expression system, yeast protein expression system, insect cell protein expression system, mammalian cell protein expression system, and prokaryotic cell-free protein expression system.

Does Tsingke provide codon optimization? Does optimization have an impact on gene expression?

Yes, Tsingke offers free codon optimization.Codon optimization enhances gene expression by using preferred codons and avoiding rare ones for a specified host. This optimization correlates strongly with improved gene expression levels. Tsingkes unique Geneoptimizer software supports optimization for tens of thousands of hosts and offers customized services. Key optimization parameters include codonpreference. GC content, restriction enzyme sites (deletion), poly structures, and sequence repeats.

What vector does Tsingke use by default?

By default, we provide pUC57 vectors containing Amp (ampicilin) for free. we also offer a library of over 200 vector options available for free.

Which sites in the pUC57 vector will the synthesized gene be cloned?

Generally, the default clone is between EcoRⅠ and Hind Ⅲ. If you have particular needs, please note and we can select other suitable cloning sites according to the experimental needs based on your sequence.

Can l send my vectors to you? Are there any requirements for my provided vector?

Yes, you can use your own vector. it is recommended to ship your vector in advance; otherwise, the turnaround time may be extended by 5 calendar days to verify the vector.We need you to ship at least 2 μg of the plasmid and provide the full sequence information of the vector.lf the full sequence is not available, please provide at least 200 bp upstream and downstream of the cloning site, along with the vector's antibiotic resistance information.

Can the plasmid delivered by gene synthesis be directly used for cell transfection?

No, the order defaults to providing 4 μg of plasmid dry powder. This plasmid is not recommended for direct transfection into cells or protein expression. If you need to conduct related experiments, please perform plasmid transformation, then isolate a single colony for cultivation, and use a plasmid extraction kit that meets the required standards to extract high-quality plasmid. Our company also provides plasmid extraction services. If you have higher requirements for the plasmid, you can select our plasmid extraction service simultaneously when placing your order.

How long can the synthesized plasmid be stored?

The synthesized plasmid can be stored for one vear. Customer-provided vectors that are free of issues will also be stored forone year. During the storage period, you can use the plasmid for site-directed mutagenesis, cloning, or other services.

What if there are quality issues with the plasmid?

If there are any quality issues within one month of receipt,we will provide a free replacement. Overseas shipping fees will be charged separately, or the plasmids can be sent with other orders.

How does Tsingke solve high-difficulty genes, such as those with highec content, highlyrepetitive sequences, verylong sequences, and special components?

High and low GC content, repetitive sequence:Solution: Divide the sequence into small segments to reduce the impact of structure, and choose the optimal connectionsolution, such as enzyme digestion and ligation and multi-segment recombination.Long sequencesSolution: use multi-segment recombination to construct divided gene fragments, and combine them with vitro and vivo assembly.Special parts of vectors (e.g. ccdB, LTR)Solution: Use different competent cells corresponding to different parts of vectors, such as DB3.1, which renders the strain resistant to the toxic effects of the ccdB gene, the recombination-deficient Stbl series, etc.Other unpredictable dlificulties, such as genetic instability and toxicity, and the introduction of random mutations and deletions.Solution: Try different competent cells.

How does Tsingke determine the quality and accuracy of the synthesized gene?

Tsingke ensures sample uniformity by isolating monoconal clones, Each clone is validated by Sanger sequencing, covering the entiregene and at least 50 bp of the vector backbone upstream and downstream, which ensures the gene is correct and properly placed inthe vector. The plasmid then undergoes NGS to verify the accuracy of the entire plasmid and detect any contamination. Beforeshipment, a strict OC review is conducted.

What deliverables are provided for Gene fragment synthesis?

A tube containing approximately 500 ng of lyophilized DNA, lectronic Certificate of Analysis (COA), sequencing chromatograms (.abi files) and Target Sequences (.seq files)if sequencing validation is needed.

What is delivered for ProLong Gene Synthesis services?

We provide one tube of lyophilized plasmid (stored at -20°C).

On which vectors are the synthesized large fragments cloned? can speclfic vectors be used?

Our company defines long DNA sequences as those exceeding 10 kb. For sequences ranging from 10 kb to 30 kb, yeastassemoly methods are usedto first construct them into the pcc1413 vector before transferring them to your specified targetvector. sequences exceeding 30 kb will be directly constructed into the pcc1413 vector.

Why choose Tsingke's Custom shRNA Service?

Efficient construction: Rapid generation of shRNA expression plasmids via gene synthesis, eliminating tedious cloning steps.Professional optimization: Customized design for any gene from any species, with sequence optimization to enhance knockdown efficiency and minimize off-target effects.Versatile vectors: A wide range of lentiviral backbones available; shRNA can be delivered by direct transfection or packaged into lentiviral particles for infection.Easy selection: Vectors carry common resistance markers (e.g., PURO/NEO) for stable cell line establishment; fluorescent markers (e.g.,GFP/mcherry) allow real-time monitoring of transfection/infection efficiency and enable flow cytometry sorting.

Does Tsingke provide viral packaging services for shRNA?

Yes, Tsingke offer end-to-end services from plasmid to ready-to-use viral particles, including packaging, concentration, and titer determination. Lentivirus, AAV, and adenovirus systems are available, delivering high-titer viral particles ready for your experiments.

What do I need to provide for PCR cloning services?

a. Templates: Provide and send us the template plasmid(≥2μg). If the plasmid was constructed by Tsingke and the order was placed within the last year, you do not need to provide it again.b. Vector details: Choose from our 200+ free vectors or provide your vector.c. Provide the sequences and resistance information for both template and vector, along with the target sequence and cloning siteinformation.d. Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fill in the relevant fields according to your needs,and send it via email to info@tsingke.com.cn to place your order.

Can I provide PCR products directly for PCR cloning?

No, the template sequence needs to be first constructed into a T-vector or another vector. then, amplify using the plasmid asthe template. Alternatively, you can choose our gene synthesis service by simply providing the DNA sequence.

What do l need to provide for site directed mutagenesis service?

a.DNA template: Please send us your templateb.Information: The sequence of the template including the target gene and vector information, mutation specifications.c.Order Form: Download the "Tsingke Gene Synthesis Order Form" from our website, fillin the relevant fields according to your needsand send it via email to info@tsingke.com.cn to place your order.

How is the pricing for site-directed mutagenesis determined? is there a charge for each mutated base?

The pricing for site-directed mutagenesis is evaluated and quoted based on the specific location of the mutation sites and the numberof mutations (all mutations within a 30-base region will be considered as one mutation).

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What is Tsingke's Plasmid Preparation Service?

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Tsingke provides high-quality plasmid extraction services with Research Grade and Transfection Grade to meet diverse experimental needs.

What do l need to provide for Plasmid Preparation service?

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We provide the following two options:

a. Gene synthesis + plasmid preparation: Synthesize your gene by Tsinkge our and then proceed with plasmid preparation.

b. Plasmid preparation only: You need to ship the target plasmid to us.

Download the "Tsingke Gene Synthesis Order Form" from our website, fillin the relevant fields according to your needs, and send it via email to info@tsingke.com.cn or gene@tsingke.com.cn to place your order.

Can the obtained plasmids be used for cell transfection?

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Yes, Tsingke provides transfection-grade plasmid preparation services with low endotoxin levels and high supercoiled DNAcontent, suitable for cell transfection, gene therapy, and gene editing. Endotoxin levels can be selected as< 0.1 Eu/μg, <0.01 EU/μg, or <0.005 EU/μg.

Why choose Tsingke's Gene to Plasmid Service?

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Tsingke offers an integrated solution from gene synthesis to plasmid preparation, ensuring high-quality plasmids delivered in a seamless and efficient workflow. This one-stop service saves time, reduces experimental complexity, and guarantees accuracy for your downstream applications.

Explore the power of the Gene Factory and discover how Tsingke's integrated platform accelerates your R&D and product development.

Tsingke Updates

2025-08-12

High-Accuracy Gene Synthesis: Overcoming GC-rich Challenges

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2025-07-31

Tsingke Biotech Hosts 2025 Small Nucleic Acid Therapeutics Roundtable in Beijing

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