DNA modificatio
Pre-validated reporter-quencher pairs for TaqMan qPCR probes, molecular beacons, and multiplexed detection — optimized for signal-to-noise and compatibility with standard instruments.
| DNA modification | |
| 5′ Modification | Internal Modification |
| 5′ Modification | ||||
| 5'Acrydite | 5'Alexa Fluor 700 | 5'C12-NH2 | 5'DIG | 5'Quasar 670 |
| 5'Alexa Flour 350 | 5'Alexa Fluor 750 | 5'C6-NH2 | 5'ET-ROX | 5'Quasar 705 |
| 5'Alexa Flour 405 | 5'AMCA | 5'C7-NH2 | 5'ET-TAMRA | 5'RHO 101 |
| 5'Alexa Flour 488 | 5'ATTO 425 | 5'CHCH | 5'FAM | 5'ROX |
| 5'Alexa Flour 532 | 5'ATTO 550 | 5'CHO | 5'Ferrocene | 5'SF670 |
| 5'Alexa Flour 546 | 5'ATTO 565 | 5'Cholesteryl | 5'FITC | 5'TAM |
| 5'Alexa Flour 555 | 5'ATTO 594 | 5'COOH | 5'HEX | 5'TET |
| 5'Alexa Flour 568 | 5'ATTO 700 | 5'CY3 | 5'JOE | 5'THL C6 SH |
| 5'Alexa Flour 594 | 5'ATTO Rho11 | 5'CY5 | 5'Maleimide | 5'THS C6 S-S |
| 5'Alexa Flour 647 | 5'Azide(N3) | 5'CY5.5 | 5'Methylene Blue | 5'triple Biotin |
| 5'Alexa Flour 680 | 5'BHQ1 | 5'CY5-M | 5'NED | 5'triple SH |
| 5'Alexa Fluor 610 | 5'BHQ2 | 5'CY7 | 5'PET | 5'TXR |
| 5'Alexa Fluor 620 | 5'BIO | 5'DAB | 5'PHO | 5'VIC |
| 5'Alexa Fluor 633 | 5'BIO-TEG | 5'DBCO | 5'Quasar 570 | 5'YakYel |
| Internal Modification | ||||
| (+A)(+C)(+G)(+T) | (dT-ATTO 550) | (dT-Quasar 570) | (dC-Alexa Flour 405) | (dC-BHQ1) |
| (5hmdC) | (dT-ATTO 565) | (dT-Quasar 670) | (dC-Alexa Flour 488) | (dC-BHQ2) |
| (C7-NH2) | (dT-ATTO 594) | (dT-Quasar 705) | (dC-Alexa Flour 532) | (dC-Biotin) |
| (-CT+Signal) | (dT-ATTO 655) | (dT-ROX) | (dC-Alexa Flour 546) | (dC-Chromeo 494) |
| (dI) | (dT-ATTO 700) | (dT-SF670) | (dC-Alexa Flour 555) | (dC-CY3) |
| (dSpacer) | (dT-ATTO Rho11) | (dT-TAM) | (dC-Alexa Flour 568) | (dC-CY5) |
| (dT-Alexa Flour 350) | (dT-Azide) | (dT-DNP) | (dC-Alexa Flour 594) | (dC-CY5-M) |
| (dT-Alexa Flour 405) | (dT-BHQ1) | (dU) | (dC-Alexa Flour 647) | (dC-Digoxin) |
| (dT-Alexa Flour 488) | (dT-BHQ2) | (m5dC) | (dC-Alexa Flour 680) | (dC-FAM) |
| (dT-Alexa Flour 532) | (dT-Biotin) | (PC Linker) | (dC-Alexa Fluor 610) | (dC-FITC) |
| (dT-Alexa Flour 546) | (dT-Chromeo 494) | (rA)(rC)(rG)(rU) | (dC-Alexa Fluor 620) | (dC-HEX) |
| (dT-Alexa Flour 555) | (dT-CY3) | (Spacer 18) | (dC-Alexa Fluor 633) | (dC-Maleimide) |
| (dT-Alexa Flour 568) | (dT-CY5) | (Spacer 9) | (dC-Alexa Fluor 700) | (dC-Methylene Blue) |
| (dT-Alexa Flour 594) | (dT-CY5-M) | (Spacer C12) | (dC-Alexa Fluor 750) | (dC-NH2) |
| (dT-Alexa Flour 647) | (dT-CY7) | (Spacer C3) | (dC-ATTO 425) | (dC-Quasar 570) |
| (dT-Alexa Flour 680) | (dT-Digoxin) | (Spacer C6) | (dC-ATTO 550) | (dC-Quasar 670) |
| (dT-Alexa Fluor 610) | (dT-FAM) | (SS) | (dC-ATTO 565) | (dC-Quasar 705) |
| (dT-Alexa Fluor 620) | (dT-FITC) | (TAO) | (dC-ATTO 594) | (dC-ROX) |
| (dT-Alexa Fluor 633) | (dT-HEX) | (XEN) | (dC-ATTO 655) | (dC-SF670) |
| (dT-Alexa Fluor 700) | (dT-Maleimide) | Phosphorothioate | (dC-ATTO 700) | (dC-TAM) |
| (dT-Alexa Fluor 750) | (dT-Methylene Blue) | ET-B/G/O/P/R/Y | (dC-ATTO Rho11) | |
| (dT-ATTO 425) | (dT-NH2) | (dC-Alexa Flour 350) | (dC-Azide) | |
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