Frequently Asked Questions

We require the DNA or amino acid sequence you need synthesized.If codon optimization is required, specify the host species. Additionally, indicate any required restriction sites at the 5' and/or 3' ends, any internal modifications to avoid, and if you need additional sequences added (e.g., Kozak sequence, stop codons, etc.).Or you can download the "Tsingke Gene Synthesis Order Form" from our website and email it to info@tsingke.com.cn.Our technical support team will promotly estimate the pricing and turnaround time for you and send you a quotation shortly.

To help us design the most effective shRNA, please provide:

Gene information: Official gene name (Gene Symbol), species or Gene ID, and the target sequence.

Vector preference: The vector you would like the shRNA sequence to be cloned into. You may select from our available vectors (based on selectable markers, etc.) or provide your own vector.

Or you can download the "Tsingke shRNA vector construction Order Form" from our website and email it to info@tsingke.com.cn. Our technical support team will promotly estimate the pricing and turnaround time for you and send you a quotation shortly.

Download the "Tsingke Gene Synthesis Order Form" from our website, complete the “Gene info”, “Cloning vector”and“Plasmid preparation”section, and send it via email to info@tsingke.com.cn to place your order.

Yes, all customers' information will be kept confidential.

Yes, Tsingke also provides protein expression and purification services for synthesized genes, offering six major protein expression systems: E. coli protein expression system, Bacillus subtilis protein expression system, yeast protein expression system, insect cell protein expression system, mammalian cell protein expression system, and prokaryotic cell-free protein expression system.

Yes, Tsingke offers free codon optimization.

Codon optimization enhances gene expression by using preferred codons and avoiding rare ones for a specified host. This optimization correlates strongly with improved gene expression levels. Tsingkes unique Geneoptimizer software supports optimization for tens of thousands of hosts and offers customized services. Key optimization parameters include codonpreference. GC content, restriction enzyme sites (deletion), poly structures, and sequence repeats.